Anti-ASGPR

Albert, J., Czaja, Dig Dis Sci, 2010

Autoimmune hepatitis (AIH) is a chronic inflammatory liver disease, with hitherto unknown origin. It ranges from a mild disease course, to progressive hepatitis with fatal outcome.

Antibodies against the asialoglycoprotein receptor (ASGPR) are described in approximately 80 % of all patients with active AIH of types I or II. In contrast to other liver specific autoantibodies, antibodies against ASGPR are a marker of histological activity of liver disease. During therapy with corticosteroids, anti-ASGPR can be used for therapeutic monitoring.

  • The only commercially available ELISA with highly purified ASGPR from rabbit liver
  • Anti-ASGPR is the only organ specific autoantibody in autoimmune liver disease
  • Anti-ASGPR allows the serological follow-up of AIH
  • Anti-ASGPR allows a differential diagnosis, to rule out toxic and other inflammatory liver diseases.

Anti-ASGPR is an enzyme immunoassay for the semi-quantitive determination of IgG antibodies against the membrane receptor of asialoglycoproteins in human serum or plasma. The analytical sensitivity is a binding index (BI) of 0.3. The diagnostic specificity, identified from a cohort of healthy blood donors, is 100 %. The values for intra- and interassay precision are in the range of 5 - 9 % (coefficient of variation).

Test Pinciple

Enzyme immunoassay, ELISA

Assay Size

96 determinations

Indication

Autoimmune liver disease

Parameter

IgG  antibodies against ASGPR

Preparation

Dilution of patient samples 1:100 with sample diluent, positive and cut-off controls are ready to use

Results

Semi-quantitative: Binding index (BI), grey area: 0.9 - 1.1 BI

Incubation Times

60 min RT -  30 min RT -  15 min RT in the dark

Substrate

Tetramethylbenzidine (TMB), 450 nm

Sample Material

Human serum or plasma

With Anti-ASGPR 96 determinations can be made within 105 minutes, which can then be evaluated semi-quantitively with a microplate photometer.

The kit is designed for both manual processing, or processing with an automated ELISA System.

Villalta, D., Mytilinaiou, M.G., Elsner, M., Hentschel, C., Cuccato J., Somma, V., Schierack, P., Roggenbuck D. and Bogdanos D.P.  Autoantibodies to asialoglycoprotein receptor (ASGPR) in patients with autoimmune liver diseases. Clin. Chim. 2015; Acta, 450, 1-5.

 

Hausdorf, G., Roggenbuck, D., Feist, E. et al. Autoantibodies to asialoglycoprotein receptor (ASGPR) measured by a novel ELISA – revival of a disease-activity marker in autoimmune hepatitis. Clin. Chim. 2009; Acta, 408, 19-24.