Anti-Gangliosid Dot Analysis of peripheral polyneuropathies with immunodot

Anti-Gangliosid Dot - 12 LINE-Profile Diagnostics (IgG/IgM)
12 LINE Profile Diagnostics (IgG/IgM)
Anti-Gangliosid Dot
Anti-Gangliosid Dot - antigen structures

Inflammatory neuropathies of the peripheral nervous system are characterized by numerous clinical symptoms, which can range from mild fatigue and uncharacteristic discomfort to neuromuscular disorders and functional deficiencies, like respiratory failure and cardiac arrest.

Autoantibodies against gangliosides occur in autoimmune diseases of the peripheral nervous system. Gangliosides are acidic glycolipids, comprised of a lipid (ceramide), an oligosaccharide chain and sialic acid.

Gangliosides are components of cell membranes and are particularly found in the central and peripheral nervous system.

These antibodies directly cause an inflammation or demyelinization of the myelin sheaths, therapy of autoimmune neuropathies is therefore based on immunoregulation.

This means that an accurate diagnosis is crucial for initiation of therapy.

The Anti-Ganglioside Dot is a membrane-based enzyme immunoassay for the determination of IgG or IgM antibodies against 12 gangliosides.

The use of a novel hydrophobic membrane ensures the optimal epitope conformation of the gangliosides, and thus gives the assay high sensitivity and specificity.

Test principleLine immunoassay
Assay size 20 determinations
IndicationPeripheral neuropathies
ParameterIgG / IgM antibodies against gangliosides (GM1, GM2, GM3, GM4, GD1a, GD1b, GD2, GD3, GT1a, GT1b, GQ1b) and sulfatide
PreparationDilution of patient samples directly in the test
Resultqualitative
Incubation Times120 min (4 °C) - 60 min (4 °C) - 10 min RT
SubstrateTetramethylbenzidine (TMB) precipitate
Sample MaterialHuman serum, plasma or CSF

With the Anti-Ganglioside Dot (5003) one analysis for a patient with peripheral neuropathy can provide a profile of 12 autoantibodies. The detection of an immune-mediated disease is therefore possible with a high probability.

In addition to manual performance, an adaption of the assay for the DotDiver is available.

Caudie, C., Quittard, Pinon A., Bouhour, F., Vial, C., Garnier, L., Fabien, N. Comparison of commercial tests for detecting multiple anti-ganglioside autoantibodies in patients with well-characterized immune-mediated peripheral neuropathies. Clin Lab. 2013; 59: 1277-87.

 

Conrad, K., Schneider, H., Ziemssen, T., Talaska, T., Reinhold, D., Humbel, R.L., Roggenbuck, D. A new line immunoassay for the multiparametric detection of antiganglioside autoantibodies in patients with autoimmune peripheral neuropathies. Ann N Y Acad Sci. 2007; 1109: 256-64.