Enzymimmunoassay (ELISA) for the quantitative determination of IgG antibodies to soluble liver antigen (SLA/LP)

Assay principle Enzyme immunoassay
Format microtiter plate coated with human recombinant SLA/LP
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 30 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

Enzyme immunoassay for the determination of IgG autoantibodies to intrinsic factor in human Serum 

Assay principle Enzyme immunoassay
Format microtiter plate coated with intrinsic factor (human recombinant)
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1 - 300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

Enzyme immunoassay for the determination of IgG autoantibodies to gastric parietal cells in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with H+/K+-ATPase, (α- und β subunit, porcine)
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1 - 300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl verdünntes Serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgA antibodies to deamidated gliadin in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with deamidated gliadin
Conjugate anti-human-IgA-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 15 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgA antibodies to glycoprotein 2 (GP-2) in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with recombinant GP-2
Conjugate anti-human-IgA-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgG antibodies to deamidated gliadin in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with deamidated gliadin
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 15 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgG antibodies to glycoprotein 2 (GP-2) in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with recombinant GP-2
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 15 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

Enzyme immunoassay for the semi-quantitative determination of IgG antibodies to asialoglycoprotein receptor in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with purified ASGPR from rabbit liver
Conjugate anti-human-IgG-HRP
Substrate TMB
Cut off 1.0 Binding index
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of glycoprotein 2 (GP-2) in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with anti-GP2 (mouse, monoclonal)
Conjugate anti-GP2 (polyclonal, rabbit) - HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 15 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl undiluted serum
Available format 96 determinations

ELISA for the quantitative and qualitative determination of IgA antibodies to Saccharomyces cerevisiae (ASCA) in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with mannan of Saccharomyces cerevisiae
Conjugate anti-human-IgA-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 20 U/ml or 1.0 Binding Index
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the quantitative and qualitative determination of IgG antibodies to Saccharomyces cerevisiae (ASCA) in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with mannan of Saccharomyces cerevisiae
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 20 U/ml or 1.0 Binding Index
Total incubation time 60-30-15 min (37°C)
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the semi-quantitative determination of IgG autoantibodies to nuclear and cytoplasmic antigens in human serum and plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with HeLa nuclei
Conjugate anti-human-IgG-HRP
Substrate TMB
Cut off 1.0 Binding index
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the semi-quantitative simultaneous determination of autoantibodies to 6 nuclear and cytoplasmic antigens in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with RNP (68kDa, A,C), Sm, SS-A (60kDa), SS-B, Scl-70, Jo-1
Conjugate anti-human-IgG-HRP
Substrate TMB
Cut off 1.0 Binding index
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 12 x 8 determinations

ELISA for the semi-quantitative separate determination of autoantibodies to 8 nuclear and cytoplasmic antigens in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with ds-DNA, RNP (68kDa, A, C), Sm, SS-A (60kDa), SS-B, Scl-70, CENP-B, Jo-1
Conjugate anti-human-IgG-HRP
Substrate TMB
Cut off 1.0 Binding index
Total incubation time 60-30-15 min
Sample volume 8 x 100 µl diluted serum 1+100 (v+v)
Available format 12 x 8 determinations

ELISA for the semi-quantitative determination of IgG, IgM and IgA antibodies (screening) to cardiolipin in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with cardiolipin and ß2 GP-I
Conjugate anti-human-IgG/IgM/IgA-HRP
Substrate TMB
Cut off 1.0 Binding index
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the qualitative and quantitative determination of IgG antibodies to double-stranded deoxyribonucleic acid (dsDNA) in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with native purified dsDNA
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 IU / ml
Cut off 35 IU/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgG or IgM antibodies to cardiolipin in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with cardiolipin and ß2 GP-I
Conjugate anti-human-IgG or IgM-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgA antibodies to Fc region of IgG in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with IgG (rabbit)
Conjugate anti-human-IgA-HRP
Substrate TMB
Standard range 1-300 U / ml
Cut off 30 U / ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgA autoantibodies to tissue transglutaminase in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with tissue transglutaminase (recombinant human) and gliadin traces
Conjugate anti-human-IgA-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 20 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the semi-quantitative determination of IgG, IgM and IgA antibodies (screening) to ß2 glycoprotein I (ß2GP-I) in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with ß2 glycoprotein I
Conjugate anti-human-IgG/IgM/IgA-HRP
Substrate TMB
Cut off 1.0 Binding index
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgG or IgM antibodies to ß2 glycoprotein I (ß2GP-I) in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with ß2 glycoprotein I
Conjugate anti-human-IgG- or -IgM-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgG autoantibodies to tissue transglutaminase in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with tissue transglutaminase (recombinant human) and gliadin traces
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 20 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgM antibodies to Fc region of IgG in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with IgG (rabbit)
Conjugate anti-human-IgM-HRP
Substrate TMB
Standard range 1–300 IU / ml
Cut off 15 IU / ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgG or IgM antibodies to phospholipids (cardiolipin, phosphatidyl-serine, - inositol, -ethanolamine, phosphatidic acid) in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with ß2 GP-I, cardiolipin, phosphatidyl-serine, - inositol, -ethanolamine and phosphatidic acid
Conjugate anti-human-IgG or IgM-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

Enzyme immunoassay for the determination of IgG antibodies to cytochrome p450 IID6 in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with human recombinant cytochrome P450 IID6 (LKM-1)
Substrate TMB
Standard range 1 - 300 U/ml
Cut off 15 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgG or IgM antibodies to phosphatidylserine in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with phosphatidylserine and ß2 glycoprotein I
Conjugate anti-human-IgG or IgM-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the qualitative and quantitative determination of IgG autoantibodies to myeloperoxidase (MPO) in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with purified human myeloperoxidase (MPO)
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the qualitative and quantitative determination of IgG autoantibodies to proteinase 3 (PR3) in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with purified human proteinase 3 (PR3)
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

Enzyme immunoassay for the determination of IgG antibodies to complement factor H in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with human recombinant, complement factor H
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1 - 300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 48 determinations

ELISA for the quantitative determination of IgG antibodies to Fc region of IgG in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with IgG (rabbit)
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 U / ml
Cut off 30 U / ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the qualitative determination of Helicobacter pylori Antigen in fecal samples

Assay principle Enzyme immunoassay (two step)
Format microtiter plate coated with anti-Helicobacter pylori antibodies (mouse monoclonal),
Conjugate anti-H pylori antibodies (mouse monoclonal) - HRP
Substrate TMB
Cut off (OD CAL2 + OD CAL1) / 2
Total incubation time 60-15 min
Sample volume 100 µl diluted stool samples
Available format 48 determinations

ELISA for the qualitative determination of Helicobacter pylori Antigen in fecal samples

Assay principle Enzyme immunoassay (two step)
Format microtiter plate coated with anti-Helicobacter pylori antibodies (mouse monoclonal),
Conjugate anti-H pylori antibodies (mouse monoclonal) - HRP
Substrate TMB
Cut off (OD CAL2 + OD CAL1) / 2
Total incubation time 60-15 min
Sample volume 100 µl diluted stool samples
Available format 96 determinations