Enzyme immunoassay for the determination of IgG autoantibodies to intrinsic factor in human Serum
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with intrinsic factor (human recombinant) |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Standard range | 1 - 300 U/ml |
Cut off | 10 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum, 1 + 100 (v+v) |
Available format | 96 determinations |
Enzyme immunoassay for the determination of IgG autoantibodies to gastric parietal cells in human serum
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with H+/K+-ATPase, (α- und β subunit, porcine) |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Standard range | 1 - 300 U/ml |
Cut off | 10 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl verdünntes Serum, 1 + 100 (v+v) |
Available format | 96 determinations |
ELISA for the quantitative determination of IgA antibodies to deamidated gliadin in human serum
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with deamidated gliadin |
Conjugate | anti-human-IgA-HRP |
Substrate | TMB |
Standard range | 1-300 U/ml |
Cut off | 15 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum, 1 + 100 (v+v) |
Available format | 96 determinations |
ELISA for the quantitative determination of IgA antibodies to glycoprotein 2 (GP-2) in human serum
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with recombinant GP-2 |
Conjugate | anti-human-IgA-HRP |
Substrate | TMB |
Standard range | 1-300 U/ml |
Cut off | 10 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum 1+100 (v+v) |
Available format | 96 determinations |
ELISA for the quantitative determination of IgG antibodies to deamidated gliadin in human serum
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with deamidated gliadin |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Standard range | 1-300 U/ml |
Cut off | 15 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum, 1 + 100 (v+v) |
Available format | 96 determinations |
ELISA for the quantitative determination of IgG antibodies to glycoprotein 2 (GP-2) in human serum
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with recombinant GP-2 |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Standard range | 1-300 U/ml |
Cut off | 15 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum 1+100 (v+v) |
Available format | 96 determinations |
Enzyme immunoassay for the semi-quantitative determination of IgG antibodies to asialoglycoprotein receptor in human serum or plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with purified ASGPR from rabbit liver |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Cut off | 1.0 Binding index |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum, 1 + 100 (v+v) |
Available format | 96 determinations |
Enzyme immunoassay for the determination of IgG antibodies to SARS-Coronavirus 2 in human serum and plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate with Spike and Nucleocapsid antigens of SARS-CoV 2 (recombinant) |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Cut off | 1.1 Binding index |
Total incubation time | 45-45-15 min (37 °C) |
Sample volume | 10 µl undiluted serum, dilution 1+20 (v+v) directly in the test |
Available format | 96 determinations |
Enzyme immunoassay for the determination of IgM antibodies to SARS-Coronavirus 2 in human serum and plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate with Spike and Nucleocapsid Antigens of SARS-CoV 2 (recombinant) |
Conjugate | anti-human-IgM-HRP |
Substrate | TMB |
Cut off | 1.1 Binding index |
Total incubation time | 45-45-15 min (37 °C) |
Sample volume | 200 µl diluted serum 1+40 (v+v) |
Available format | 96 determinations |
Enzyme immunoassay for the determination of the IgG antibodies specificity to immunodominant antigens of SARS-Coronavirus 2 in human serum and plasma previously tested positive for IgG antibodies
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate separately coated with Spike Glycoprotein 1, Spike Glycoprotein 2 and Nucleocapsid antigens of SARS-CoV 2 (recombinant) |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Cut off | 1.2 Binding index |
Total incubation time | 45-45-15 min (37 °C) |
Sample volume | 2x 200 µl diluted serum 1+20 (v+v) |
Available format | 24 x 3 determinations |
ELISA for the quantitative determination of glycoprotein 2 (GP-2) in human serum
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with anti-GP2 (mouse, monoclonal) |
Conjugate | anti-GP2 (polyclonal, rabbit) - HRP |
Substrate | TMB |
Standard range | 1-300 U/ml |
Cut off | 15 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl undiluted serum |
Available format | 96 determinations |
ELISA for the quantitative and qualitative determination of IgA antibodies to Saccharomyces cerevisiae (ASCA) in human serum
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with mannan of Saccharomyces cerevisiae |
Conjugate | anti-human-IgA-HRP |
Substrate | TMB |
Standard range | 1-300 U/ml |
Cut off | 20 U/ml or 1.0 Binding Index |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum, 1 + 100 (v+v) |
Available format | 96 determinations |
ELISA for the quantitative and qualitative determination of IgG antibodies to Saccharomyces cerevisiae (ASCA) in human serum
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with mannan of Saccharomyces cerevisiae |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Standard range | 1-300 U/ml |
Cut off | 20 U/ml or 1.0 Binding Index |
Total incubation time | 60-30-15 min (37°C) |
Sample volume | 100 µl diluted serum, 1 + 100 (v+v) |
Available format | 96 determinations |
ELISA for the semi-quantitative determination of IgG autoantibodies to nuclear and cytoplasmic antigens in human serum and plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with HeLa nuclei |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Cut off | 1.0 Binding index |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum 1+100 (v+v) |
Available format | 96 determinations |
ELISA for the semi-quantitative separate determination of autoantibodies to 8 nuclear and cytoplasmic antigens in human serum or plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with ds-DNA, RNP (68kDa, A, C), Sm, SS-A (60kDa), SS-B, Scl-70, CENP-B, Jo-1 |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Cut off | 1.0 Binding index |
Total incubation time | 60-30-15 min |
Sample volume | 8 x 100 µl diluted serum 1+100 (v+v) |
Available format | 12 x 8 determinations |
ELISA for the semi-quantitative determination of IgG, IgM and IgA antibodies (screening) to cardiolipin in human serum or plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with cardiolipin and ß2 GP-I |
Conjugate | anti-human-IgG/IgM/IgA-HRP |
Substrate | TMB |
Cut off | 1.0 Binding index |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum 1+100 (v+v) |
Available format | 96 determinations |
ELISA for the qualitative and quantitative determination of IgG antibodies to double-stranded deoxyribonucleic acid (dsDNA) in human serum or plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with native purified dsDNA |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Standard range | 1-300 IU / ml |
Cut off | 35 IU/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum 1+100 (v+v) |
Available format | 96 determinations |
ELISA for the quantitative determination of IgG or IgM antibodies to cardiolipin in human serum or plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with cardiolipin and ß2 GP-I |
Conjugate | anti-human-IgG or IgM-HRP |
Substrate | TMB |
Standard range | 1-300 U/ml |
Cut off | 10 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum 1+100 (v+v) |
Available format | 96 determinations |
ELISA for the quantitative determination of IgA antibodies to Fc region of IgG in human serum or plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with IgG (rabbit) |
Conjugate | anti-human-IgA-HRP |
Substrate | TMB |
Standard range | 1-300 U / ml |
Cut off | 30 U / ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum 1+100 (v+v) |
Available format | 96 determinations |
ELISA for the quantitative determination of IgA autoantibodies to tissue transglutaminase in human serum
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with tissue transglutaminase (recombinant human) and gliadin traces |
Conjugate | anti-human-IgA-HRP |
Substrate | TMB |
Standard range | 1-300 U/ml |
Cut off | 20 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum, 1 + 100 (v+v) |
Available format | 96 determinations |
ELISA for the semi-quantitative determination of IgG, IgM and IgA antibodies (screening) to ß2 glycoprotein I (ß2GP-I) in human serum or plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with ß2 glycoprotein I |
Conjugate | anti-human-IgG/IgM/IgA-HRP |
Substrate | TMB |
Cut off | 1.0 Binding index |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum 1+100 (v+v) |
Available format | 96 determinations |
ELISA for the quantitative determination of IgG or IgM antibodies to ß2 glycoprotein I (ß2GP-I) in human serum or plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with ß2 glycoprotein I |
Conjugate | anti-human-IgG- or -IgM-HRP |
Substrate | TMB |
Standard range | 1-300 U/ml |
Cut off | 10 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum 1+100 (v+v) |
Available format | 96 determinations |
ELISA for the quantitative determination of IgG autoantibodies to tissue transglutaminase in human serum
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with tissue transglutaminase (recombinant human) and gliadin traces |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Standard range | 1-300 U/ml |
Cut off | 20 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum, 1 + 100 (v+v) |
Available format | 96 determinations |
ELISA for the quantitative determination of IgM antibodies to Fc region of IgG in human serum or plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with IgG (rabbit) |
Conjugate | anti-human-IgM-HRP |
Substrate | TMB |
Standard range | 1–300 IU / ml |
Cut off | 15 IU / ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum 1+100 (v+v) |
Available format | 96 determinations |
ELISA for the quantitative determination of IgG or IgM antibodies to phospholipids (cardiolipin, phosphatidyl-serine, - inositol, -ethanolamine, phosphatidic acid) in human serum or plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with ß2 GP-I, cardiolipin, phosphatidyl-serine, - inositol, -ethanolamine and phosphatidic acid |
Conjugate | anti-human-IgG or IgM-HRP |
Substrate | TMB |
Standard range | 1-300 U/ml |
Cut off | 10 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum 1+100 (v+v) |
Available format | 96 determinations |
Enzyme immunoassay for the determination of IgG antibodies to cytochrome p450 IID6 in human serum or plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with human recombinant cytochrome P450 IID6 (LKM-1) |
Substrate | TMB |
Standard range | 1 - 300 U/ml |
Cut off | 15 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum, 1 + 100 (v+v) |
Available format | 96 determinations |
ELISA for the quantitative determination of IgG or IgM antibodies to phosphatidylserine in human serum or plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with phosphatidylserine and ß2 glycoprotein I |
Conjugate | anti-human-IgG or IgM-HRP |
Substrate | TMB |
Standard range | 1-300 U/ml |
Cut off | 10 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum 1+100 (v+v) |
Available format | 96 determinations |
ELISA for the qualitative and quantitative determination of IgG autoantibodies to myeloperoxidase (MPO) in human serum
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with purified human myeloperoxidase (MPO) |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Standard range | 1-300 U/ml |
Cut off | 10 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum, 1 + 100 (v+v) |
Available format | 96 determinations |
ELISA for the qualitative and quantitative determination of IgG autoantibodies to proteinase 3 (PR3) in human serum
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with purified human proteinase 3 (PR3) |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Standard range | 1-300 U/ml |
Cut off | 10 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum, 1 + 100 (v+v) |
Available format | 96 determinations |
Enzyme immunoassay for the determination of IgG antibodies to complement factor H in human serum
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with human recombinant, complement factor H |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Standard range | 1 - 300 U/ml |
Cut off | 10 U/ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum, 1 + 100 (v+v) |
Available format | 48 determinations |
ELISA for the quantitative determination of IgG antibodies to Fc region of IgG in human serum or plasma
Assay principle | Enzyme immunoassay |
---|---|
Format | microtiter plate coated with IgG (rabbit) |
Conjugate | anti-human-IgG-HRP |
Substrate | TMB |
Standard range | 1-300 U / ml |
Cut off | 30 U / ml |
Total incubation time | 60-30-15 min |
Sample volume | 100 µl diluted serum 1+100 (v+v) |
Available format | 96 determinations |
ELISA for the qualitative determination of Helicobacter pylori Antigen in fecal samples
Assay principle | Enzyme immunoassay (two step) |
---|---|
Format | microtiter plate coated with anti-Helicobacter pylori antibodies (mouse monoclonal), |
Conjugate | anti-H pylori antibodies (mouse monoclonal) - HRP |
Substrate | TMB |
Cut off | (OD CAL2 + OD CAL1) / 2 |
Total incubation time | 60-15 min |
Sample volume | 100 µl diluted stool samples |
Available format | 48 determinations |
ELISA for the qualitative determination of Helicobacter pylori Antigen in fecal samples
Assay principle | Enzyme immunoassay (two step) |
---|---|
Format | microtiter plate coated with anti-Helicobacter pylori antibodies (mouse monoclonal), |
Conjugate | anti-H pylori antibodies (mouse monoclonal) - HRP |
Substrate | TMB |
Cut off | (OD CAL2 + OD CAL1) / 2 |
Total incubation time | 60-15 min |
Sample volume | 100 µl diluted stool samples |
Available format | 96 determinations |