Enzyme immunoassays (EIA)

    Enzyme immunoassay for the determination of IgG autoantibodies to intrinsic factor in human Serum 

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with intrinsic factor (human recombinant)
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Standard range 1 - 300 U/ml
    Cut off 10 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µl diluted serum, 1 + 100 (v+v)
    Available format 96 determinations

    Enzyme immunoassay for the determination of IgG autoantibodies to gastric parietal cells in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with H+/K+-ATPase, (α- und β subunit, porcine)
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Standard range 1 - 300 U/ml
    Cut off 10 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µl verdünntes Serum, 1 + 100 (v+v)
    Available format 96 determinations

    Enzyme linked immunosorbent assay (ELISA) for the quantitative determination of IgG antibodies to cyclic citrullinated peptide (CCP) in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with CCP antigens

    ELISA for the quantitative determination of IgA antibodies to deamidated gliadin in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with deamidated gliadin
    Conjugate anti-human-IgA-HRP
    Substrate TMB
    Standard range 1-300 U/ml
    Cut off 15 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µl diluted serum, 1 + 100 (v+v)
    Available format 96 determinations

    ELISA for the quantitative determination of IgA antibodies to glycoprotein 2 (GP-2) in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with recombinant GP-2
    Conjugate anti-human-IgA-HRP
    Substrate TMB
    Standard range 1-300 U/ml
    Cut off 10 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µl diluted serum 1+100 (v+v)
    Available format 96 determinations

    ELISA for the quantitative determination of IgG antibodies to deamidated gliadin in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with deamidated gliadin
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Standard range 1-300 U/ml
    Cut off 15 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µl diluted serum, 1 + 100 (v+v)
    Available format 96 determinations

    ELISA for the quantitative determination of IgG antibodies to glycoprotein 2 (GP-2) in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with recombinant GP-2
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Standard range 1-300 U/ml
    Cut off 15 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µl diluted serum 1+100 (v+v)
    Available format 96 determinations

    Enzyme immunoassay for the semi-quantitative determination of IgG antibodies to asialoglycoprotein receptor in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with purified ASGPR from rabbit liver
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Cut off 1.0 Binding index
    Total incubation time 60-30-15 min
    Sample volume 100 µL diluted serum, 1 + 100 (v+v)
    Available format 96 determinations

    Enzyme immunoassay for the determination of IgG antibodies to SARS-Coronavirus 2 in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate with Spike and Nucleocapsid antigens of SARS-CoV 2 (recombinant)
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Cut off 1.1 Binding index
    Total incubation time 45-45-15 min (37 °C)
    Sample volume 10 µL undiluted serum, dilution 1+20 (v+v) directly in the test
    Available format 96 determinations

    Enzyme immunoassay for the determination of IgM antibodies to SARS-Coronavirus 2 in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate with Spike and Nucleocapsid Antigens of SARS-CoV 2 (recombinant)
    Conjugate anti-human-IgM-HRP
    Substrate TMB
    Cut off 1.1 Binding index
    Total incubation time 45-45-15 min (37 °C)
    Sample volume 200 µL diluted serum 1+40 (v+v)
    Available format 96 determinations

    Enzyme immunoassay for the determination of the IgG antibodies specificity to immunodominant antigens of SARS-Coronavirus 2 in human serum previously tested positive for IgG antibodies

    Assay principle Enzyme immunoassay
    Format microtiter plate separately coated with Spike Glycoprotein 1, Spike Glycoprotein 2 and Nucleocapsid antigens of SARS-CoV 2 (recombinant)
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Cut off 1.2 Binding index
    Total incubation time 45-45-15 min (37 °C)
    Sample volume 2x 200 µL diluted serum 1+20 (v+v)
    Available format 24 x 3 determinations

    ELISA for the quantitative determination of glycoprotein 2 (GP-2) in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with anti-GP2 (mouse, monoclonal)
    Conjugate anti-GP2 (polyclonal, rabbit) - HRP
    Substrate TMB
    Standard range 1-300 U/ml
    Cut off 15 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µl undiluted serum
    Available format 96 determinations

    ELISA for the quantitative and qualitative determination of IgA antibodies to Saccharomyces cerevisiae (ASCA) in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with mannan of Saccharomyces cerevisiae
    Conjugate anti-human-IgA-HRP
    Substrate TMB
    Standard range 1-300 U/ml
    Cut off 20 U/ml or 1.0 Binding Index
    Total incubation time 60-30-15 min
    Sample volume 100 µl diluted serum, 1 + 100 (v+v)
    Available format 96 determinations

    ELISA for the quantitative and qualitative determination of IgG antibodies to Saccharomyces cerevisiae (ASCA) in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with mannan of Saccharomyces cerevisiae
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Standard range 1-300 U/ml
    Cut off 20 U/ml or 1.0 Binding Index
    Total incubation time 60-30-15 min (37°C)
    Sample volume 100 µl diluted serum, 1 + 100 (v+v)
    Available format 96 determinations

    ELISA for the semi-quantitative determination of IgG autoantibodies to nuclear and cytoplasmic antigens in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with HeLa nuclei
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Cut off 1.0 Binding index
    Total incubation time 60-30-15 min
    Sample volume 100 µL diluted serum 1+100 (v+v)
    Available format 96 determinations

    ELISA for the semi-quantitative separate determination of autoantibodies to 8 nuclear and cytoplasmic antigens in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with ds-DNA, RNP (68kDa, A, C), Sm, SS-A (60kDa), SS-B, Scl-70, CENP-B, Jo-1
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Cut off 1.0 Binding index
    Total incubation time 60-30-15 min
    Sample volume 8 x 100 µL diluted serum 1+100 (v+v)
    Available format 12 x 8 determinations

    ELISA for the semi-quantitative determination of IgG, IgM and IgA antibodies (screening) to cardiolipin in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with cardiolipin and ß2 GP-I
    Conjugate anti-human-IgG/IgM/IgA-HRP
    Substrate TMB
    Cut off 1.0 Binding index
    Total incubation time 60-30-15 min
    Sample volume 100 µL diluted serum 1+100 (v+v)
    Available format 96 determinations

    ELISA for the qualitative and quantitative determination of IgG antibodies to double-stranded deoxyribonucleic acid (dsDNA) in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with native purified dsDNA
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Standard range 1-300 IU / ml
    Cut off 35 IU/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µL diluted serum 1+100 (v+v)
    Available format 96 determinations

    ELISA for the quantitative determination of IgG or IgM antibodies to cardiolipin in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with cardiolipin and ß2 GP I
    Conjugate anti-human-IgG or IgM-HRP
    Substrate TMB
    Standard range 1-300 U/ml
    Cut off 10 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µL diluted serum 1+100 (v+v)
    Available format 96 determinations

    ELISA for the quantitative determination of IgA antibodies to Fc region of IgG in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with IgG (rabbit)
    Conjugate anti-human-IgA-HRP
    Substrate TMB
    Standard range 1-300 U / ml
    Cut off 30 U / ml
    Total incubation time 60-30-15 min
    Sample volume 100 µL diluted serum 1+100 (v+v)
    Available format 96 determinations

    ELISA for the quantitative determination of IgA autoantibodies to tissue transglutaminase in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with tissue transglutaminase (recombinant human) and gliadin traces
    Conjugate anti-human-IgA-HRP
    Substrate TMB
    Standard range 1-300 U/ml
    Cut off 20 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µl diluted serum, 1 + 100 (v+v)
    Available format 96 determinations

    ELISA for the semi-quantitative determination of IgG, IgM and IgA antibodies (screening) to ß2 glycoprotein-I (ß2GP-I) in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with ß2 glycoprotein-I
    Conjugate anti-human-IgG/IgM/IgA-HRP
    Substrate TMB
    Cut off 1.0 Binding index
    Total incubation time 60-30-15 min
    Sample volume 100 µL diluted serum 1+100 (v+v)
    Available format 96 determinations

    ELISA for the quantitative determination of IgG or IgM antibodies to ß2 glycoprotein-I (ß2GP-I) in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with ß2 glycoprotein-I
    Conjugate anti-human-IgG- or -IgM-HRP
    Substrate TMB
    Standard range 1-300 U/ml
    Cut off 10 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µL diluted serum 1+100 (v+v)
    Available format 96 determinations

    ELISA for the quantitative determination of IgG autoantibodies to tissue transglutaminase in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with tissue transglutaminase (recombinant human) and gliadin traces
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Standard range 1-300 U/ml
    Cut off 20 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µL diluted serum, 1 + 100 (v+v)
    Available format 96 determinations

    ELISA for the quantitative determination of IgM antibodies to Fc region of IgG in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with IgG (rabbit)
    Conjugate anti-human-IgM-HRP
    Substrate TMB
    Standard range 1–300 IU / ml
    Cut off 15 IU / ml
    Total incubation time 60-30-15 min
    Sample volume 100 µL diluted serum 1+100 (v+v)
    Available format 96 determinations

    ELISA for the quantitative determination of IgG or IgM antibodies to phospholipids (cardiolipin, phosphatidyl-serine, - inositol, -ethanolamine, phosphatidic acid) in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with ß2 GP-I, cardiolipin, phosphatidyl-serine, - inositol, -ethanolamine and phosphatidic acid
    Conjugate anti-human-IgG or IgM-HRP
    Substrate TMB
    Standard range 1-300 U/ml
    Cut off 10 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µL diluted serum 1+100 (v+v)
    Available format 96 determinations

    Enzyme immunoassay for the determination of IgG antibodies to cytochrome p450 IID6 in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with human recombinant cytochrome P450 IID6 (LKM-1)
    Substrate TMB
    Standard range 1 - 300 U/ml
    Cut off 15 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µL diluted serum, 1 + 100 (v+v)
    Available format 96 determinations

    ELISA for the quantitative determination of IgG or IgM antibodies to phosphatidylserine in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with phosphatidylserine and ß2 glycoprotein-I
    Conjugate anti-human-IgG or IgM-HRP
    Substrate TMB
    Standard range 1-300 U/ml
    Cut off 10 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µL diluted serum 1+100 (v+v)
    Available format 96 determinations

    ELISA for the qualitative and quantitative determination of IgG autoantibodies to myeloperoxidase (MPO) in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with purified human myeloperoxidase (MPO)
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Standard range 1-300 U/ml
    Cut off 10 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µl diluted serum, 1 + 100 (v+v)
    Available format 96 determinations

    ELISA for the qualitative and quantitative determination of IgG autoantibodies to proteinase 3 (PR3) in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with purified human proteinase 3 (PR3)
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Standard range 1-300 U/ml
    Cut off 10 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µl diluted serum, 1 + 100 (v+v)
    Available format 96 determinations

    Enzyme immunoassay for the determination of IgG antibodies to complement factor H in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with human recombinant, complement factor H
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Standard range 1 - 300 U/ml
    Cut off 10 U/ml
    Total incubation time 60-30-15 min
    Sample volume 100 µl diluted serum, 1 + 100 (v+v)
    Available format 48 determinations

    ELISA for the quantitative determination of IgG antibodies to Fc region of IgG in human serum

    Assay principle Enzyme immunoassay
    Format microtiter plate coated with IgG (rabbit)
    Conjugate anti-human-IgG-HRP
    Substrate TMB
    Standard range 1-300 U / ml
    Cut off 30 U / ml
    Total incubation time 60-30-15 min
    Sample volume 100 µL diluted serum 1+100 (v+v)
    Available format 96 determinations

    ELISA for the qualitative determination of Helicobacter pylori Antigen in fecal samples

    Assay principle Enzyme immunoassay (two step)
    Format microtiter plate coated with anti-Helicobacter pylori antibodies (mouse monoclonal),
    Conjugate anti-H pylori antibodies (mouse monoclonal) - HRP
    Substrate TMB
    Cut off (OD CAL2 + OD CAL1) / 2
    Total incubation time 60-15 min
    Sample volume 100 µl diluted stool samples
    Available format 48 determinations

    ELISA for the qualitative determination of Helicobacter pylori Antigen in fecal samples

    Assay principle Enzyme immunoassay (two step)
    Format microtiter plate coated with anti-Helicobacter pylori antibodies (mouse monoclonal),
    Conjugate anti-H pylori antibodies (mouse monoclonal) - HRP
    Substrate TMB
    Cut off (OD CAL2 + OD CAL1) / 2
    Total incubation time 60-15 min
    Sample volume 100 µl diluted stool samples
    Available format 96 determinations