ANA/ENA/ANCA
Indirect immunofluorescence assay for the determination of autoantibodies to nuclear and cytoplasmic antigens in human serum
Assay principle | Indirect immunofluorescence assay (IIF) |
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Format | slides coated with HEp-2 cells |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 25 µl diluted serum |
Available format | 120 (10 x 12) determinations |
Indirect immunofluorescence assay for the determination of autoantibodies to nuclear and cytoplasmic antigens in human serum
Assay principle | Indirect immunofluorescence assay (IIF) |
---|---|
Format | slides coated with HEp-2 cells |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 25 µl diluted serum |
Available format | 480 (40 x 12) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies to native DNA in human serum
Assay principle | Indirect immunofluorescence assay |
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Format | slides coated with Crithidia luciliae cells |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 25 µl diluted serum |
Available format | 60 (10 x 6) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies to native DNA in human serum
Assay principle | Indirect immunofluorescence assay |
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Format | slides coated with Crithidia luciliae cells |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 25 µl diluted serum |
Available format | 120 (10 x 12) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies to neutrophil cytoplasmic antigens (ANCA) human serum
Assay principle | Indirect immunofluorescence assay |
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Format | slides coated with human granulocytes, ethanol fixed |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 25 µl diluted serum |
Available format | 60 (10 x 6) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies to neutrophil cytoplasmic antigens (ANCA) human serum
Assay principle | Indirect immunofluorescence assay |
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Format | slides coated with human granulocytes, formalin fixed |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 25 µl diluted serum |
Available format | 60 (10 x 6) determinations |
CytoBead® Tests
Indirect immunfluorescence assay for the determination of IgG antibodies against neutrophil cytoplasmic antigens (ANCA) and glomerular basement membrane (GBM) in human serum
Assay principle | Indirect immunofluorescence assay |
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Format | slides coated with human granulocytes (ethanol fixed) and beads coated with PR3, MPO and GBM |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 60 min |
Sample volume | 50 µl diluted serum |
Available format | 48 (6 x 8) determinations |
Indirect immunofluorescence assay for the determination of IgA or IgG antibodies against endomysium (EmA), transglutaminase 2 and deamidated gliadin as well for the control of total IgA antibodies in human serum
Assay principle | Indirect immunofluorescence (IIF) |
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Format | slides with monkey tissue sections (esophagus) and beads coated with TG2, DG and anti-IgA |
Conjugate | anti-human-IgA-FITC, anti-human-IgG-FITC |
Total incubation time | 60 min |
Sample volume | 50 µl diluted serum |
Available format | 48 (6 x 8) determinations |
Indirect immunofluorescence assay for the determination of autoantibodies to nuclear and cytoplasmic antigens in human serum
Assay principle | Indirect immunofluorescence assay |
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Format | slides coated with HEp-2 cells and beads coated with dsDNA, Scl-70, SS-A/Ro60, SS-A/Ro52, SS-B, CENP-B, Sm, Sm/RNP |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 60 min |
Sample volume | 80 µl diluted serum |
Available format | 80 (10 x 8) determinations |
Downloads
- CytoBead® ANA /ANA 2 271 KB
Indirect immunofluorescence assay for the determination of IgG antibodies against neutrophil cytoplasmic antigens (ANCA), the glomerular basement membrane (GBM) and double stranded deoxyribonucleic acid (dsDNA) in human serum
Assay principle | Indirect immunofluorescence (IIF) |
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Format | slides coated with human granulocytes (ethanol fixed) and beads coated with PR3, MPO, GBM and dsDNA |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 60 min |
Sample volume | 50 µl diluted serum |
Available format | 48 (6 x 8) determinations |
Indirect immunofluorescence assay for the determination of autoantibodies to nuclear and cytoplasmic antigens in human serum
Assay principle | Indirect immunofluorescence assay |
---|---|
Format | slides coated with HEp-2 cells and beads coated with dsDNA, Scl-70, SS-A/ Ro60, SS-A/Ro52, SS-B, Jo-1, Sm, Sm/RNP) |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 60 min |
Sample volume | 80 µl diluted serum |
Available format | 80 (10 x 8) determinations |
Tissue sections
Indirect immunofluorescence assay for the determination of anti-mitochondrial antibodies (AMA, polyvalent) in human serum
Assay principle | Indirect immunofluorescence (IIF) |
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Format | slides with tissue sections (rat kidney) |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 50 µl diluted serum |
Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of antibodies to the smooth muscle (ASMA, polyvalent) in human serum
Assay principle | Indirect immunofluorescence (IIF) |
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Format | slides with tissue sections (rat stomach) |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 50 µl diluted serum |
Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of autoantibodies (ANA/AMA/ASMA/APCA) in human serum
Assay principle | Indirect immunofluorescence (IIF) |
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Format | slides with rat tissue sections (liver / stomach / kidney) |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 50 µl diluted serum |
Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of autoantibodies (ANA/AMA/ASMA/APCA) in human serum
Assay principle | Indirect immunofluorescence (IIF) |
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Format | slides with rat tissue sections (liver / stomach / kidney) |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 50 µl diluted serum |
Available format | 96 (12 x 8) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies against adrenal cortex (AAA) in human serum
Assay principle | Indirect immunofluorescence (IIF) |
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Format | slides with monkey tissue sections (adrenal cortex) |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 50 µl diluted serum |
Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies against Islet cells (ICA) in human serum
Assay principle | Indirect immunofluorescence (IIF) |
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Format | slides with monkey tissue sections (pancreas) |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 50 µl diluted serum |
Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of IgA antibodies against endomysium (EmA) in human serum
Assay principle | Indirect immunofluorescence (IIF) |
---|---|
Format | slides with monkey tissue sections (esophagus) |
Conjugate | anti-human-IgA-FITC |
Total incubation time | 30-30 min |
Sample volume | 50 µl diluted serum |
Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of IgA antibodies against endomysium (EmA) in human serum
Assay principle | Indirect immunofluorescence (IIF) |
---|---|
Format | slides with monkey tissue sections (esophagus) |
Conjugate | anti-human-IgA-FITC |
Total incubation time | 30-30 min |
Sample volume | 50 µl diluted serum |
Available format | 96 (12 x 8) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies against skin antigens (ASA) in human serum
Assay principle | Indirect immunofluorescence (IIF) |
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Format | slides with monkey tissue sections (esophagus) |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 50 µl diluted serum |
Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies against cardiac muscle antigens (CMA) in human serum
Assay principle | Indirect immunofluorescence (IIF) |
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Format | slides with monkey tissue sections (heart muscle) |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 50 µl diluted serum |
Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies against skeletal muscle antigens (SkMA) in human serum
Assay principle | Indirect immunofluorescence (IIF) |
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Format | slides with monkey tissue sections (skeletal muscle) |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 50 µl diluted serum |
Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies against the glomerular basal membrane (GBM) in human serum
Assay principle | Indirect immunofluorescence (IIF) |
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Format | slides with monkey tissue sections (kidney) |
Conjugate | anti-human-IgG-FITC |
Total incubation time | 30-30 min |
Sample volume | 50 µl diluted serum |
Available format | 48 (12 x 4) determinations |