ANA/ENA/ANCA

Indirect immunofluorescence assay for the determination of autoantibodies to nuclear and cytoplasmic antigens in human serum

Assay principle Indirect immunofluorescence assay (IIF)
Format slides coated with HEp-2 cells
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 25 µl diluted serum
Available format 120 (10 x 12) determinations

Indirect immunofluorescence assay for the determination of autoantibodies to nuclear and cytoplasmic antigens in human serum

Assay principle Indirect immunofluorescence assay (IIF)
Format slides coated with HEp-2 cells
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 25 µl diluted serum
Available format 480 (40 x 12) determinations

Indirect immunofluorescence assay for the determination of IgG antibodies to native DNA in human serum

Assay principle Indirect immunofluorescence assay
Format slides coated with Crithidia luciliae cells
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 25 µl diluted serum
Available format 60 (10 x 6) determinations

Indirect immunofluorescence assay for the determination of IgG antibodies to native DNA in human serum

Assay principle Indirect immunofluorescence assay
Format slides coated with Crithidia luciliae cells
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 25 µl diluted serum
Available format 120 (10 x 12) determinations

Indirect immunofluorescence assay for the determination of IgG antibodies to neutrophil cytoplasmic antigens (ANCA) human serum

Assay principle Indirect immunofluorescence assay
Format slides coated with human granulocytes, ethanol fixed
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 25 µl diluted serum
Available format 60 (10 x 6) determinations

Indirect immunofluorescence assay for the determination of IgG antibodies to neutrophil cytoplasmic antigens (ANCA) human serum

Assay principle Indirect immunofluorescence assay
Format slides coated with human granulocytes, formalin fixed
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 25 µl diluted serum
Available format 60 (10 x 6) determinations

CytoBead® Tests

Indirect immunfluorescence assay for the determination of IgG antibodies against neutrophil cytoplasmic antigens (ANCA) and glomerular basement membrane (GBM) in human serum

Assay principle Indirect immunofluorescence assay
Format slides coated with human granulocytes (ethanol fixed) and beads coated with PR3, MPO and GBM
Conjugate anti-human-IgG-FITC
Total incubation time 60 min
Sample volume 50 µl diluted serum
Available format 48 (6 x 8) determinations

Indirect immunofluorescence assay for the determination of IgA or IgG antibodies against endomysium (EmA), transglutaminase 2 and deamidated gliadin as well for the control of total IgA antibodies in human serum

Assay principle Indirect immunofluorescence (IIF)
Format slides with monkey tissue sections (esophagus) and beads coated with TG2, DG and anti-IgA
Conjugate anti-human-IgA-FITC, anti-human-IgG-FITC
Total incubation time 60 min
Sample volume 50 µl diluted serum
Available format 48 (6 x 8) determinations

Indirect immunofluorescence assay for the determination of autoantibodies to nuclear and cytoplasmic antigens in human serum

Assay principle Indirect immunofluorescence assay
Format slides coated with HEp-2 cells and beads coated with dsDNA, Scl-70, SS-A/Ro60, SS-A/Ro52, SS-B, CENP-B, Sm, Sm/RNP
Conjugate anti-human-IgG-FITC
Total incubation time 60 min
Sample volume 80 µl diluted serum
Available format 80 (10 x 8) determinations

Indirect immunofluorescence assay for the determination of IgG antibodies against neutrophil cytoplasmic antigens (ANCA), the glomerular basement membrane (GBM) and double stranded deoxyribonucleic acid (dsDNA) in human serum 

Assay principle Indirect immunofluorescence (IIF)
Format slides coated with human granulocytes (ethanol fixed) and beads coated with PR3, MPO, GBM and dsDNA
Conjugate anti-human-IgG-FITC
Total incubation time 60 min
Sample volume 50 µl diluted serum
Available format 48 (6 x 8) determinations

Indirect immunofluorescence assay for the determination of autoantibodies to nuclear and cytoplasmic antigens in human serum

Assay principle Indirect immunofluorescence assay
Format slides coated with HEp-2 cells and beads coated with dsDNA, Scl-70, SS-A/ Ro60, SS-A/Ro52, SS-B, Jo-1, Sm, Sm/RNP)
Conjugate anti-human-IgG-FITC
Total incubation time 60 min
Sample volume 80 µl diluted serum
Available format 80 (10 x 8) determinations

Tissue sections

Indirect immunofluorescence assay for the determination of anti-mitochondrial antibodies (AMA, polyvalent) in human serum

Assay principle Indirect immunofluorescence (IIF)
Format slides with tissue sections (rat kidney)
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 50 µl diluted serum
Available format 48 (12 x 4) determinations

Indirect immunofluorescence assay for the determination of antibodies to the smooth muscle (ASMA, polyvalent) in human serum

Assay principle Indirect immunofluorescence (IIF)
Format slides with tissue sections (rat stomach)
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 50 µl diluted serum
Available format 48 (12 x 4) determinations

Indirect immunofluorescence assay for the determination of autoantibodies (ANA/AMA/ASMA/APCA) in human serum

Assay principle Indirect immunofluorescence (IIF)
Format slides with rat tissue sections (liver / stomach / kidney)
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 50 µl diluted serum
Available format 48 (12 x 4) determinations

Indirect immunofluorescence assay for the determination of autoantibodies (ANA/AMA/ASMA/APCA) in human serum

Assay principle Indirect immunofluorescence (IIF)
Format slides with rat tissue sections (liver / stomach / kidney)
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 50 µl diluted serum
Available format 96 (12 x 8) determinations

Indirect immunofluorescence assay for the determination of IgG antibodies against adrenal cortex (AAA) in human serum

Assay principle Indirect immunofluorescence (IIF)
Format slides with monkey tissue sections (adrenal cortex)
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 50 µl diluted serum
Available format 48 (12 x 4) determinations

Indirect immunofluorescence assay for the determination of IgG antibodies against Islet cells (ICA) in human serum 

Assay principle Indirect immunofluorescence (IIF)
Format slides with monkey tissue sections (pancreas)
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 50 µl diluted serum
Available format 48 (12 x 4) determinations

Indirect immunofluorescence assay for the determination of IgA antibodies against endomysium (EmA) in human serum

Assay principle Indirect immunofluorescence (IIF)
Format slides with monkey tissue sections (esophagus)
Conjugate anti-human-IgA-FITC
Total incubation time 30-30 min
Sample volume 50 µl diluted serum
Available format 48 (12 x 4) determinations

Indirect immunofluorescence assay for the determination of IgA antibodies against endomysium (EmA) in human serum

Assay principle Indirect immunofluorescence (IIF)
Format slides with monkey tissue sections (esophagus)
Conjugate anti-human-IgA-FITC
Total incubation time 30-30 min
Sample volume 50 µl diluted serum
Available format 96 (12 x 8) determinations

Indirect immunofluorescence assay for the determination of IgG antibodies against skin antigens (ASA) in human serum

Assay principle Indirect immunofluorescence (IIF)
Format slides with monkey tissue sections (esophagus)
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 50 µl diluted serum
Available format 48 (12 x 4) determinations

Indirect immunofluorescence assay for the determination of IgG antibodies against cardiac muscle antigens (CMA) in human serum

Assay principle Indirect immunofluorescence (IIF)
Format slides with monkey tissue sections (heart muscle)
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 50 µl diluted serum
Available format 48 (12 x 4) determinations

Indirect immunofluorescence assay for the determination of IgG antibodies against skeletal muscle antigens (SkMA) in human serum

Assay principle Indirect immunofluorescence (IIF)
Format slides with monkey tissue sections (skeletal muscle)
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 50 µl diluted serum
Available format 48 (12 x 4) determinations

Indirect immunofluorescence assay for the determination of IgG antibodies against the glomerular basal membrane (GBM) in human serum

Assay principle Indirect immunofluorescence (IIF)
Format slides with monkey tissue sections (kidney)
Conjugate anti-human-IgG-FITC
Total incubation time 30-30 min
Sample volume 50 µl diluted serum
Available format 48 (12 x 4) determinations