ANA/ENA/ANCA
Indirect immunofluorescence assay for the determination of autoantibodies to nuclear and cytoplasmic antigens in human serum
| Assay principle | Indirect immunofluorescence assay (IIF) |
|---|---|
| Format | slides coated with HEp-2 cells |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 25 µl diluted serum |
| Available format | 120 (10 x 12) determinations |
Indirect immunofluorescence assay for the determination of autoantibodies to nuclear and cytoplasmic antigens in human serum
| Assay principle | Indirect immunofluorescence assay (IIF) |
|---|---|
| Format | slides coated with HEp-2 cells |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 25 µl diluted serum |
| Available format | 480 (40 x 12) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies to native DNA in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | slides coated with Crithidia luciliae cells |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 25 µl diluted serum |
| Available format | 60 (10 x 6) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies to native DNA in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | slides coated with Crithidia luciliae cells |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 25 µl diluted serum |
| Available format | 120 (10 x 12) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies to neutrophil cytoplasmic antigens (ANCA) human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | slides coated with human granulocytes, ethanol fixed |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 25 µl diluted serum |
| Available format | 60 (10 x 6) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies to neutrophil cytoplasmic antigens (ANCA) human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | slides coated with human granulocytes, formalin fixed |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 25 µl diluted serum |
| Available format | 60 (10 x 6) determinations |
CytoBead® Tests
Indirect immunfluorescence assay for the determination of IgG antibodies against neutrophil cytoplasmic antigens (ANCA) and glomerular basement membrane (GBM) in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | slides coated with human granulocytes (ethanol fixed) and beads coated with PR3, MPO and GBM |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 min |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (6 x 8) determinations |
Indirect immunofluorescence assay for the determination of IgA or IgG antibodies against endomysium (EmA), transglutaminase 2 and deamidated gliadin as well for the control of total IgA antibodies in human serum
| Assay principle | Indirect immunofluorescence (IIF) |
|---|---|
| Format | slides with monkey tissue sections (esophagus) and beads coated with TG2, DG and anti-IgA |
| Conjugate | anti-human-IgA-FITC, anti-human-IgG-FITC |
| Total incubation time | 60 min |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (6 x 8) determinations |
Indirect immunofluorescence assay for the determination of autoantibodies to nuclear and cytoplasmic antigens in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | slides coated with HEp-2 cells and beads coated with dsDNA, Scl-70, SS-A/Ro60, SS-A/Ro52, SS-B, CENP-B, Sm, Sm/RNP |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 min |
| Sample volume | 80 µl diluted serum |
| Available format | 80 (10 x 8) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies against neutrophil cytoplasmic antigens (ANCA), the glomerular basement membrane (GBM) and double stranded deoxyribonucleic acid (dsDNA) in human serum
| Assay principle | Indirect immunofluorescence (IIF) |
|---|---|
| Format | slides coated with human granulocytes (ethanol fixed) and beads coated with PR3, MPO, GBM and dsDNA |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 min |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (6 x 8) determinations |
Indirect immunofluorescence assay for the determination of autoantibodies to nuclear and cytoplasmic antigens in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | slides coated with HEp-2 cells and beads coated with dsDNA, Scl-70, SS-A/ Ro60, SS-A/Ro52, SS-B, Jo-1, Sm, Sm/RNP) |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 min |
| Sample volume | 80 µl diluted serum |
| Available format | 80 (10 x 8) determinations |
Tissue sections
Indirect immunofluorescence assay for the determination of anti-mitochondrial antibodies (AMA, polyvalent) in human serum
| Assay principle | Indirect immunofluorescence (IIF) |
|---|---|
| Format | slides with tissue sections (rat kidney) |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of antibodies to the smooth muscle (ASMA, polyvalent) in human serum
| Assay principle | Indirect immunofluorescence (IIF) |
|---|---|
| Format | slides with tissue sections (rat stomach) |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of autoantibodies (ANA/AMA/ASMA/APCA) in human serum
| Assay principle | Indirect immunofluorescence (IIF) |
|---|---|
| Format | slides with rat tissue sections (liver / stomach / kidney) |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of autoantibodies (ANA/AMA/ASMA/APCA) in human serum
| Assay principle | Indirect immunofluorescence (IIF) |
|---|---|
| Format | slides with rat tissue sections (liver / stomach / kidney) |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 50 µl diluted serum |
| Available format | 96 (12 x 8) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies against adrenal cortex (AAA) in human serum
| Assay principle | Indirect immunofluorescence (IIF) |
|---|---|
| Format | slides with monkey tissue sections (adrenal cortex) |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies against Islet cells (ICA) in human serum
| Assay principle | Indirect immunofluorescence (IIF) |
|---|---|
| Format | slides with monkey tissue sections (pancreas) |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of IgA antibodies against endomysium (EmA) in human serum
| Assay principle | Indirect immunofluorescence (IIF) |
|---|---|
| Format | slides with monkey tissue sections (esophagus) |
| Conjugate | anti-human-IgA-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of IgA antibodies against endomysium (EmA) in human serum
| Assay principle | Indirect immunofluorescence (IIF) |
|---|---|
| Format | slides with monkey tissue sections (esophagus) |
| Conjugate | anti-human-IgA-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 50 µl diluted serum |
| Available format | 96 (12 x 8) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies against skin antigens (ASA) in human serum
| Assay principle | Indirect immunofluorescence (IIF) |
|---|---|
| Format | slides with monkey tissue sections (esophagus) |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies against cardiac muscle antigens (CMA) in human serum
| Assay principle | Indirect immunofluorescence (IIF) |
|---|---|
| Format | slides with monkey tissue sections (heart muscle) |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies against skeletal muscle antigens (SkMA) in human serum
| Assay principle | Indirect immunofluorescence (IIF) |
|---|---|
| Format | slides with monkey tissue sections (skeletal muscle) |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12 x 4) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies against the glomerular basal membrane (GBM) in human serum
| Assay principle | Indirect immunofluorescence (IIF) |
|---|---|
| Format | slides with monkey tissue sections (kidney) |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 30-30 min |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12 x 4) determinations |
