Indication Anti phospholipid syndrome

ELISA for the semi-quantitative determination of IgG, IgM and IgA antibodies (screening) to cardiolipin in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with cardiolipin and ß2 GP-I
Conjugate anti-human-IgG/IgM/IgA-HRP
Substrate TMB
Cut off 1.0 Binding index
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgG or IgM antibodies to cardiolipin in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with cardiolipin and ß2 GP-I
Conjugate anti-human-IgG or IgM-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the semi-quantitative determination of IgG, IgM and IgA antibodies (screening) to ß2 glycoprotein I (ß2GP-I) in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with ß2 glycoprotein I
Conjugate anti-human-IgG/IgM/IgA-HRP
Substrate TMB
Cut off 1.0 Binding index
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgG or IgM antibodies to ß2 glycoprotein I (ß2GP-I) in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with ß2 glycoprotein I
Conjugate anti-human-IgG- or -IgM-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgG or IgM antibodies to phospholipids (cardiolipin, phosphatidyl-serine, - inositol, -ethanolamine, phosphatidic acid) in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with ß2 GP-I, cardiolipin, phosphatidyl-serine, - inositol, -ethanolamine and phosphatidic acid
Conjugate anti-human-IgG or IgM-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgG or IgM antibodies to phosphatidylserine in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with phosphatidylserine and ß2 glycoprotein I
Conjugate anti-human-IgG or IgM-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

Immunodots for the determination of  IgG- or IgM antibodies to phospholipids and ß2- glycoprotein I in human serum

Assay principle Line / Dot Immunoassay
Format membrane coated with phosphatidylserin (bovin), phosphatidylinositol (bovin), cardiolipin (bovin), ß2- glycoprotein I (human)
Conjugate anti-human-IgG-HRP, anti-human-IgM-HRP
Substrate TMB
Total incubation time 30-15-10 min
Sample volume 30 µl neat serum
Available format 20 x 4 determinations

Immunodots for the determination of  IgG- or IgM antibodies to phospholipids and serum proteins in human serum

Assay principle Line / Dot Immunoassay
Format membrane coated with highly purified antigens: cardiolipin, phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, annexin V, ß2GPI, prothrombin
Conjugate anti-human-IgG-HRP, anti-human-IgM-HRP
Substrate TMB
Total incubation time 30-15-10 min
Sample volume 30 µl neat serum
Available format 20 x 10 determinations