Indication Autoimmune hepatitis

Enzymimmunoassay (ELISA) for the quantitative determination of IgG antibodies to soluble liver antigen (SLA/LP)

Assay principle Enzyme immunoassay
Format microtiter plate coated with human recombinant SLA/LP
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 30 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

Enzyme immunoassay for the semi-quantitative determination of IgG antibodies to asialoglycoprotein receptor in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with purified ASGPR from rabbit liver
Conjugate anti-human-IgG-HRP
Substrate TMB
Cut off 1.0 Binding index
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

Immunodot for the qualitative determination of IgG antibodies to M2, LKM1, LC1 and SLA in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with M2, LKM1, LC1 (human recombinant) and SLA (rat)
Conjugate anti-human-IgG-AP
Substrate NBT/BCIP
Total incubation time 10-30-30-10 min
Sample volume 10 µl neat serum
Available format 24 x 4 determinations

Immunodot for the qualitative determination of IgG antibodies to M2, LKM1, LC1, SLA, and F-actin in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with M2, LKM1, LC1 (human recombinant), SLA (rat) and F-actin (rabbit)
Conjugate anti-human-IgG-AP
Substrate NBT/BCIP
Total incubation time 10-30-30-10 min
Sample volume 10 µl neat serum
Available format 24 x 5 determinations

Enzyme immunoassay for the determination of IgG antibodies to cytochrome p450 IID6 in human serum or plasma

Assay principle Enzyme immunoassay
Format microtiter plate coated with human recombinant cytochrome P450 IID6 (LKM-1)
Substrate TMB
Standard range 1 - 300 U/ml
Cut off 15 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

Immunodot for the qualitative determination of IgG antibodies to M2, LKM1, LC1, SLA, F-actin, gp210 and sp100 in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with M2, LKM1, LC1, gp210, sp100 (human recombinant), SLA (rat) and F-actin (rabbit)
Conjugate anti-human-IgG-AP
Substrate NBT/BCIP
Total incubation time 10-30-30-10 min
Sample volume 10 µl neat serum
Available format 24 x 7 determinations