Celiac disease / Crohn’s disease / Pancreatitis

ELISA for the quantitative determination of IgA antibodies to deamidated gliadin in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with deamidated gliadin
Conjugate anti-human-IgA-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 15 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgA antibodies to glycoprotein 2 (GP-2) in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with recombinant GP-2
Conjugate anti-human-IgA-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgG antibodies to deamidated gliadin in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with deamidated gliadin
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 15 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgG antibodies to glycoprotein 2 (GP-2) in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with recombinant GP-2
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 15 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum 1+100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of glycoprotein 2 (GP-2) in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with anti-GP2 (mouse, monoclonal)
Conjugate anti-GP2 (polyclonal, rabbit) - HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 15 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl undiluted serum
Available format 96 determinations

ELISA for the quantitative and qualitative determination of IgA antibodies to Saccharomyces cerevisiae (ASCA) in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with mannan of Saccharomyces cerevisiae
Conjugate anti-human-IgA-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 20 U/ml or 1.0 Binding Index
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the quantitative and qualitative determination of IgG antibodies to Saccharomyces cerevisiae (ASCA) in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with mannan of Saccharomyces cerevisiae
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 20 U/ml or 1.0 Binding Index
Total incubation time 60-30-15 min (37°C)
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgA autoantibodies to tissue transglutaminase in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with tissue transglutaminase (recombinant human) and gliadin traces
Conjugate anti-human-IgA-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 20 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the quantitative determination of IgG autoantibodies to tissue transglutaminase in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with tissue transglutaminase (recombinant human) and gliadin traces
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 20 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

Line Immuno Assay zur Bestimmung von IgG Antikörpern gegen deamidiertes Gliadin und Gewebstransglutaminase in humanem Serum oder Plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with tissue transglutaminase, (human recombinant) and deamidated gliadin
Conjugate anti-human-IgG-HRP
Substrate TMB
Total incubation time 10-30-30-10 min
Sample volume 10 µl neat serum
Available format 20 x 2 Bestimmungen

Line Immunoassay for the determination of IgA antibodies to deamidated gliadin and tissue transglutaminase in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with tissue transglutaminase, (human recombinant) and deamidated gliadin
Conjugate anti-human-IgA-HRP
Substrate TMB
Total incubation time 10-30-30-10 min
Sample volume 10 µl neat serum
Available format 20 x 2 determinations

Line Immunoassay for the determination of IgG and/or IgA antibodies to deamidated gliadin, tissue transglutaminase, intrinsic factor, parietal cell antigens and Saccharomyces cerevisae in human serum or plasma

Assay principle Line / Dot Immunoassay
Format Membrane coated with intrinsic factor, parietal cell antigen H+/K+ ATPase, deamidated gliadin, tissue transglutaminase and mannan from saccharomyces cerevisiae
Conjugate anti-human-IgA POD, anti-human IgG POD, anti-human IgA/IgG POD
Substrate TMB
Total incubation time 10-30-30-10 min
Sample volume 10 µl natives Serum
Available format 20 x 5 determinations