Indication Pernicious anemia

Enzyme immunoassay for the determination of IgG autoantibodies to intrinsic factor in human Serum 

Assay principle Enzyme immunoassay
Format microtiter plate coated with intrinsic factor (human recombinant)
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1 - 300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

Enzyme immunoassay for the determination of IgG autoantibodies to gastric parietal cells in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with H+/K+-ATPase, (α- und β subunit, porcine)
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1 - 300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl verdünntes Serum, 1 + 100 (v+v)
Available format 96 determinations

Line immunoassay for the qualitative determination of IgG autoantibodies to intrinsic factor and parietal cell antigen in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with intrinsic factor, H+/K+ ATPase (porcine)
Conjugate anti-human-IgG-HRP
Substrate TMB
Total incubation time 5-30-30-10 min
Sample volume 20 µl neat serum
Available format 20 x 2 determinations