Indication Vasculitis / Nephritis

Immunodot for the qualitative determination of IgG autoantibodies to Myeloperoxidase (MPO), Proteinase 3 (PR3) and Glomerular Basement Membrane protein (GBM) in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with MPO, PR3 (native human) and GBM protein NC1 3 (IV) (recombinant)
Conjugate anti-human-IgG-AP
Substrate NBT/BCIP
Total incubation time 10-30-30-10 min
Sample volume 10 µl neat serum
Available format 24 x 3 determinations

ELISA for the qualitative and quantitative determination of IgG autoantibodies to myeloperoxidase (MPO) in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with purified human myeloperoxidase (MPO)
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations

ELISA for the qualitative and quantitative determination of IgG autoantibodies to proteinase 3 (PR3) in human serum

Assay principle Enzyme immunoassay
Format microtiter plate coated with purified human proteinase 3 (PR3)
Conjugate anti-human-IgG-HRP
Substrate TMB
Standard range 1-300 U/ml
Cut off 10 U/ml
Total incubation time 60-30-15 min
Sample volume 100 µl diluted serum, 1 + 100 (v+v)
Available format 96 determinations