LINE/Dot

Immunodot for the qualitative determination of IgG autoantibodies to Myeloperoxidase (MPO), Proteinase 3 (PR3) and Glomerular Basement Membrane protein (GBM) in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with MPO, PR3 (native human) and GBM protein NC1 3 (IV) (recombinant)
Conjugate anti-human-IgG-AP
Substrate NBT/BCIP
Total incubation time 10-30-30-10 min
Sample volume 10 µl neat serum
Available format 24 x 3 determinations

Immunodot for the qualitative determination of IgG antibodies to M2, LKM1, LC1 and SLA in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with M2, LKM1, LC1 (human recombinant) and SLA (rat)
Conjugate anti-human-IgG-AP
Substrate NBT/BCIP
Total incubation time 10-30-30-10 min
Sample volume 10 µl neat serum
Available format 24 x 4 determinations

Immunodot for the qualitative determination of IgG antibodies to M2, LKM1, LC1, SLA, and F-actin in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with M2, LKM1, LC1 (human recombinant), SLA (rat) and F-actin (rabbit)
Conjugate anti-human-IgG-AP
Substrate NBT/BCIP
Total incubation time 10-30-30-10 min
Sample volume 10 µl neat serum
Available format 24 x 5 determinations

Immunodot for the separate determination of autoantibodies to nuclear and cytoplasmic antigens in human serum or plasma

Format membrane coated with Jo-1, PL-7, PL-12, SRP, PMScl, Ku, Mi-2, Scl-70
Conjugate anti-human-IgG-AP
Substrate NBT/BCIP
Total incubation time 10-30-30-10 min
Sample volume 10 µl neat serum
Available format 24 x 8 determinations

Immunodot for the separate determination of autoantibodies to nuclear and cytoplasmic antigens in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with Sm, Sm/RNP, SS-B, Jo-1, Scl-70 (bovine), SS-A, PMScl, Ku, CENP, PCNA (human recombinant)
Conjugate anti-human-IgG-AP
Substrate NBT/BCIP
Total incubation time 10-30-30-10 min
Sample volume 10 µl neat serum
Available format 24 x 10 determinations

Immunodot for the separate determination of autoantibodies to nuclear and cytoplasmic antigens in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with Sm, Sm/RNP, SS-B, Jo-1, Scl-70 (bovine), SS-A, (human recombinant)
Conjugate anti-human-IgG-AP
Substrate NBT/BCIP
Total incubation time 10-30-30-10 min
Sample volume 10 µl neat serum
Available format 24 x 6 determinations

Immunodot for the qualitative determination of IgG antibodies to M2, LKM1, LC1, SLA, F-actin, gp210 and sp100 in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with M2, LKM1, LC1, gp210, sp100 (human recombinant), SLA (rat) and F-actin (rabbit)
Conjugate anti-human-IgG-AP
Substrate NBT/BCIP
Total incubation time 10-30-30-10 min
Sample volume 10 µl neat serum
Available format 24 x 7 determinations

Line Immuno Assay zur Bestimmung von IgG Antikörpern gegen deamidiertes Gliadin und Gewebstransglutaminase in humanem Serum oder Plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with tissue transglutaminase, (human recombinant) and deamidated gliadin
Conjugate anti-human-IgG-HRP
Substrate TMB
Total incubation time 10-30-30-10 min
Sample volume 10 µl neat serum
Available format 20 x 2 Bestimmungen

Line Immunoassay for the determination of IgA antibodies to deamidated gliadin and tissue transglutaminase in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with tissue transglutaminase, (human recombinant) and deamidated gliadin
Conjugate anti-human-IgA-HRP
Substrate TMB
Total incubation time 10-30-30-10 min
Sample volume 10 µl neat serum
Available format 20 x 2 determinations

Line immunoassay for the qualitative determination of IgG autoantibodies to intrinsic factor and parietal cell antigen in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with intrinsic factor, H+/K+ ATPase (porcine)
Conjugate anti-human-IgG-HRP
Substrate TMB
Total incubation time 5-30-30-10 min
Sample volume 20 µl neat serum
Available format 20 x 2 determinations

Immunodot for the separate determination of autoantibodies to nuclear and cytoplasmic antigens in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with dsDNA, nucleosomes,
Conjugate anti-human-IgG-HPR
Substrate TMB
Total incubation time 5-30-30-10 min
Sample volume 20 µl neat serum
Available format 20 x 12 determinations

Line Immunoassay for the determination of IgG and/or IgA antibodies to deamidated gliadin, tissue transglutaminase, intrinsic factor, parietal cell antigens and Saccharomyces cerevisae in human serum or plasma

Assay principle Line / Dot Immunoassay
Format Membrane coated with intrinsic factor, parietal cell antigen H+/K+ ATPase, deamidated gliadin, tissue transglutaminase and mannan from saccharomyces cerevisiae
Conjugate anti-human-IgA POD, anti-human IgG POD, anti-human IgA/IgG POD
Substrate TMB
Total incubation time 10-30-30-10 min
Sample volume 10 µl natives Serum
Available format 20 x 5 determinations

Immunodot for qualitative determination of IgG or IgM antibodies to gangliosides in human serum

Assay principle Line / Dot Immunoassay
Format membranes coated with GM1, GM2, GM3, GM4, GD1a, GD1b, GD2, GD3, GT1a, GT1b, GQ1b, sulfatides
Conjugate anti-human-IgG-HRP, anti-human-IgM-HRP
Substrate TMB
Total incubation time 120 (4°C)-60 (4°C)-10
Sample volume 10 µl neat serum
Available format 20x12 determinations

Immunodots for the determination of  IgG- or IgM antibodies to phospholipids and ß2- glycoprotein I in human serum

Assay principle Line / Dot Immunoassay
Format membrane coated with phosphatidylserin (bovin), phosphatidylinositol (bovin), cardiolipin (bovin), ß2- glycoprotein I (human)
Conjugate anti-human-IgG-HRP, anti-human-IgM-HRP
Substrate TMB
Total incubation time 30-15-10 min
Sample volume 30 µl neat serum
Available format 20 x 4 determinations

Immunodots for the determination of  IgG- or IgM antibodies to phospholipids and serum proteins in human serum

Assay principle Line / Dot Immunoassay
Format membrane coated with highly purified antigens: cardiolipin, phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, annexin V, ß2GPI, prothrombin
Conjugate anti-human-IgG-HRP, anti-human-IgM-HRP
Substrate TMB
Total incubation time 30-15-10 min
Sample volume 30 µl neat serum
Available format 20 x 10 determinations

Immunodot for the qualitative determination of IgA antibodies to deamidated gliadin and autoantibodies to tissue transglutaminase in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with tissue transglutaminase, (human recombinant) and deamidated gliadin
Available format 24 x 2 determinations

Immunodot for the qualitative determination of IgG antibodies to deamidated gliadin and autoantibodies to tissue transglutaminase in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with tissue transglutaminase, (human recombinant) and deamidated gliadin
Available format 24 x 2 determinations

Immunodot for the separate determination of autoantibodies to nuclear and cytoplasmic antigens in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with Sm, Sm/RNP, SS-A, SS-B, Jo-1, Scl-70, PM-Scl-100 and CENP-A/B
Available format 24 x 7 determinations

Immunodot for the separate determination of autoantibodies to nuclear and cytoplasmic antigens in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with Jo-1, Scl-70 (bovine), PL-7, PL-12, PMScl, Mi2, Ku (human recombinant)
Available format 24 x 8 determinations

Immunodot for the qualitative determination of IgG autoantibodies to Myeloperoxidase (MPO), Proteinase 3 (PR3) and Glomerular Basement Membrane protein (GBM) in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with MPO, PR3 (native human) and GBM protein NC1 3(IV) (recombinant)
Available format 24 x 3 determinations

Immunodot for the qualitative determination of IgG autoantibodies to intrinsic factor and parietal cell antigen in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with intrinsic factor, H+/K+ ATPase (porcine)
Available format 24 x 2 determinations

Immunodots for the determination of IgG antibodies to Nucleosome, dsDNA, Histones, Sm, RNP (68kD/A/C), Sm/RNP, SSA/Ro 60kD, SSA/Ro 52kD, SSB, Scl-70, Ku, PM-Scl 100, Mi-2, Jo-1, PL-7, PL-12, SRP, Ribosomes, CENP-A/B, PCNA, sp100, gp210, M2 recombinant, M2/nPDC and F-actin in human serum

Assay principle Line / Dot Immunoassay
Format membrane coated with nucleosome, dsDNA, histone, Sm, RNP (68kD/A/C), Sm/RNP, SSA/Ro 60kD, SSA/Ro 52kD, SSB, Scl-70, Ku, PM-Scl 100, Mi-2, Jo-1, PL-7, PL-12, SRP, Ribosomes, CENP-A/B, PCNA, sp100, gp210, M2 recombinant, M2/nPDC and F-actin
Available format 24 determinations

Immunodot for the qualitative determination of IgG antibodies to M2, LKM1, LC1, SLA, F-actin, gp210 and sp100 in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with M2, LKM1, LC1, gp210, sp100 (human recombinant), SLA (rat) and F-actin (rabbit)
Available format 24 x 7 determinations

Immunodot for the automated determination of IgG antibodies to Nucleosome, Histone, Sm and Ribosome in human serum or plasma with the DotDiver

Assay principle Line / Dot Immunoassay
Format membrane coated with Nucleosome, Histone, Sm and Ribosome
Available format 24x4 determinations

Immunodot for the qualitative determination of IgG/IgM autoantibodies to gangliosides in human serum or plasma 

Assay principle Line / Dot Immunoassay
Format membrane coated with sulfatides (bovine), GM1, GM2, GM3, GM4, GD1a, GD1b, GD2, GD3, GT1a, GT1b, and GQ1b (human)
Available format 20 x 12 determinations

Immunodot for the determination of IgG antibodies to nuclear and cytoplasmic antigens in human serum or plasma with the DotDiver

Assay principle Line / Dot Immunoassay
Format membrane coated with Sm, RNP, Sm/RNP, SS-A, SS-B, Jo-1, Scl-70, PM-Scl, Ku, CENP-A/B, PCNA and Ribosome P0
Available format 24 x 12 determinations

Immunodot for the qualitative determination of IgG autoantibodies to gangliosides in human serum or plasma 

Assay principle Line / Dot Immunoassay
Format membrane coated with sulfatides (bovine), GM1, GM2, GM3, GM4, GD1a, GD1b, GD2, GD3, GT1a, GT1b, and GQ1b (human)
Available format 20 x 12 determinations

Immunodot for the qualitative determination of IgM autoantibodies to gangliosides in human serum or plasma 

Assay principle Line / Dot Immunoassay
Format membrane coated with sulfatides (bovine), GM1, GM2, GM3, GM4, GD1a, GD1b, GD2, GD3, GT1a, GT1b, and GQ1b (human)
Available format 20 x 12 determinations

Immunodot for the automated determination of IgG antibodies to phospholipids and serum proteins in human serum or plasma 

Assay principle Line / Dot Immunoassay
Format membrane coated with cardiolipin, phosphatidic acid, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine,annexin V, ß2GPI, prothrombin
Available format 20 x 9 determinations

Immunodot for the automated determination of IgM antibodies to phospholipids and serum proteins in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with cardiolipin, phosphatidic acid, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine,annexin V, ß2GPI, prothrombin
Available format 20 x 9 determinations

Immunodot for the determination of IgG antibodies to nuclear and cytoplasmic antigens in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with Jo-1, PL-7, PL-12, EJ, SRP-54, Mi-2, MDA-5, TIF1-γ, Ku, PM-Scl 100, Scl-70 and SSA/Ro52
Available format 24 x 12 determinations

Immunodot for the determination of IgG antibodies to Jo-1, PL-7, PL-12, EJ, OJ, KS, ZO, HA, SRP and P0 in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with Jo-1, PL-7, PL-12, EJ, OJ, KS, ZO, HA, SRP, P0
Available format 24 x 10 determinations

Immunodot for the automated determination of IgG antibodies to Scl-70, CENP-A, CENP-B, PM/Scl-100, PM/Scl-75, Ku, RNA Polymerase III, RNP (68kD/A/C), Fibrillarin and Th/To in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with Scl-70, CENP-A, CENP-B, PM/Scl-100, PM/Scl-75, Ku, RNA Polymerase III, RNP (68kD/A/C), Fibrillarin, Th/To
Available format 24 x 10 determinations

Immunodot for the automated determination of IgG antibodies to M2/nPDC, M2/OGDC-E2, M2/BCOADC-E2, M2/PDC-E2, gp210, sp100, LKM1, LC1, SLA and F-actin in human serum or Plasma   

Assay principle Line / Dot Immunoassay
Format membrane coated with M2-nPDC, PDC-E2, OGDC-E2, BCOADC-E2, sp100, gp210, LKM1, LC1(human recombinant), SLA (rat) and F-actin (rabbit)
Available format 24 x 10 determinations

System for fully automatic processing and interpretation of immunodots

Immunodot for the automated determination of IgG antibodies to Jo-1, PL-7, PL-12, EJ, SRP, Mi-2, MDA-5, TIF1-γ, SSA/Ro52kD, SAE1, SAE2 and NXP-2 in human serum or plasma

Assay principle Line / Dot Immunoassay
Format membrane coated with Jo-1, PL-7, PL-12, EJ, SRP, Mi-2, MDA-5, TIF1-γ, SSA/Ro52kD, SAE1, SAE2, NXP-2
Available format 24 x 12 determinations